The auxotrophic mutant could be used as a selectable marker in plant transformations.
2.
This technique is often used in industrial amino acid productions with the auxotrophic mutants.
3.
In a microbial process employing an auxotrophic mutant ( nutritionally requiring mutant ), excess supply of the required nutrient results in abundant cell growth with little accumulation of the desired metabolite due to feedback inhibition and / or end-product repression.
4.
In this assay, a naturally competent tryptophan auxotrophic mutant of " Acinetobacter baylyi " ( BD4 trpE27 ) is transformed with the total DNA of a putative " Acinetobacter " isolate and the transformation mixture is plated on a brain heart infusion agar.
5.
Lenski chose to carry out the experiment with the bacteria grown in a glucose-limited minimal medium called DM25, which was initially developed by Bernard Davis for use in isolating auxotrophic mutants of " E . coli " using penicillin as a selective agent.
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